By R. Fani, M. Bazzicalupo, E. Gallori, L. Turbanti, M. Polsinelli (auth.), Prof. Dr. Walter Klingmüller (eds.)
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Extra info for Azospirillum III: Genetics · Physiology · Ecology Proceedings of the Third Bayreuth Azospirillum Workshop
Plasmid ONA was isolated as described by Rodriguez and Tait (18). Large quantities of plasmid ONA were obtained by the eleared lysate method followed by a final purification in a CsCI-Ethbr equilibrium density-gradient centrigugation (19). Nick translatian and ONA-ONA hybridization: Preparations of pure restriction fragments were labelled with (~_32p)dCTP by nick translatian (19,20). Hybridization was don e as previously described (19). In vitro packaging: The procedures of preparing packaging extraets and of in vitro packaging followed the outlines recently published (12,21).
Acad. Sci. USA 76, 2866-2870 16. Cannon, F. , Dixon, R. , Postgate, J. R. B. (1974). Mo!. Gen. Genet. 174, 59-66 17. Marmur, J. (1961). J. Mo!. Bio!. ~, 208-218 18. Rodriguez, R. L. and Tait, R. C. (1983). Recombinant DNA techniques. An introduction. Addison-Wesley publishing company, London 19. Singh, M. and KlingmOller, W. In: Trends in molecular gen~ tics. U. Sinha and W. ) Spectrum Publishing House, Patna, Delhi, pp. 15-34, (in press) 20. , Rhodes, C. and Berg, P. (1977). J. Mol. Biol. 113, 237-251 21.
153, 45-56 CONSTRUCTION OF A GENOMIC LIBRARY OF AZOSPIRILLUM BRASILENSE SP7 AND CLONING OF THE GLUTAMINE SYNTHETASE GENE C. Fogher, H. K. Bandhari and C. Elmerich Unite de Physiologie Cellulaire, Departement de Biochimie et Genetique Moleculaire, Institut Pasteur, 28 rue du Dr. Roux 75724 Paris Cedex 15, France Summary: A gene library of total DNA of ~. brasilense Sp7 was constructed in the broad host range cosmid vector pVK100. A plasmid, designated pAB44, that restored by complementation a Gln+ Nif+ phenotype to the Gln- Nif- A.
Azospirillum III: Genetics · Physiology · Ecology Proceedings of the Third Bayreuth Azospirillum Workshop by R. Fani, M. Bazzicalupo, E. Gallori, L. Turbanti, M. Polsinelli (auth.), Prof. Dr. Walter Klingmüller (eds.)